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Sensing probes are chemical compounds that respond to the presence of a given molecule with a specific change of relaxation properties. The molecules of interest include enzymes and various low-molecular weight analytes. Using sensing probes the laboratory is actively developing novel strategies of enzyme-mediated magnetic resonance imaging signal amplification (MRamp). This strategy is based on the effect of on-site polymerization of paramagnetic substrates and their retention at the disease site. We previously developed macrocyclic and open-chelate types of oxidoreductase-specific paramagnetic substrates. One of the substrates, di-5-hydroxytryptamide of DTPA(Gd), di-5-HT-DTPAGd, is now available for investigational research on a multigram scale. The use of “enzyme sensing” MR contrast agents is expected to have a broad utility in high throughput screening as well as for in vivo detection of antigen expression patterns (Funded by NIBIB RO1 grant). Our laboratory is focusing on two major applications: 1. Imaging of cancer-specific molecules. 2. Detection of endogenous and exogenous oxidoreductases (e.g. myeloperoxidase) in cardiovascular disease and cancer.
Collaborators: Dr. John W. Chen, Massachusetts General Hospital Dr. Ajay Wakhloo, UMASS Dr. Matt Gounis, UMASS Dr. Chris Sotak, Worcester Polytechnic Institute Dr. Jeff Tsao, Novartis Research Institute
Recent Publications: Querol M, Bennett DG, Sotak C, Kang, HW, Bogdanov A Jr. A Paramagnetic Contrast Agent for Detecting Tyrosinase Activity. Chembiochem 2007 8: 1637-41. Chen JW, Querol Sans M, Bogdanov, A Jr, Weissleder R. Imaging inflammation: a multimodality molecular imaging approach targeting myeloperoxidase. Radiology 2006, 240:473-481. Querol M, Chen JW, Bogdanov A Jr. A paramagnetic contrast agent with myeloperoxidase-sensing properties Org Biomol Chem, 2006, 4:1887-1895. |
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MR Imaging of L6-positive tumor after injection of peroxidase-sensing contrast agent. L6 was targeted using antibody-enzyme conjugates. Interantional Society of Magnetic Resonance in MedicineŠ |
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