Transcription factor imaging probes

Reporters of gene transcription in cancer cells potentially could be used in both cancer phenotyping and staging, as well as for evaluating new therapies.

Recently, we developed novel oligonucleotide molecular reporters (ODMR) that release fluorescence signals upon the specific binding of transcription factor/regulator protein to ODMR encoded nucleotide sequence (Funded by NCI technology developement grant).

The future goals of our research:

1. optimization and characterization of the far-red fluorochrome labeled ODMRs

2. investigation of transcription-factor reporting properties.

Collaborators:

Dr. Anand Kumar (A. Martinos Center MGH)

Dr. David Tabatadze (MGH)

Dr. Remy Fagard (Hospital Avicenne, Paris)

Recent Publications:

Zhang S, Bogdanov A. Plate Capture Assay of Fluorescent Oligonucleotide Duplex Reporter-Transcription Factor Complexes. Bioconj. Chem 2009, 20: 1444-48.

Zhang S, Metelev V, Tabatadze D, Zamecnik P, Bogdanov A Jr. Near-infrared fluorescent oligonucleotides reporters for sensing NF-kappaB interactions in vitro. Oligonucleotides 2008, 18:235-43.

Zhang S, Metelev V, Tabatadze D, Zamecnik P, Bogdanov A Jr. Fluorescence resonance energy transfer in near-infrared fluorescent oligonucleotide probes for detecting protein-DNA interactions. Proc. Natl.Acad Sci USA 2008 105: 4156-4161

Tabatadze D, Zamecnik P, Yanachkov I, Wright G, Pierson K, Zhang S, Bogdanov A Jr., Metelev V. A novel thymidine phosphoramidite synthon for incorporation of internucleoside phosphate linkers during automated oligodeoxynucleotide synthesisde synthesis. NN&NA 2008 27:157-72.

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 A dot surface model of an oligonucleotide hairpin probe with interacting fluorochrome and quencher on opposite termini Manuel Querol ©